Ya Zhang - Ministry of Education, Pharmacy Shihezi University Xinjiang China
Changsheng Yuan - Ministry of Education, Pharmacy Shihezi University Xinjiang China
He Fang - Ministry of Education, Pharmacy Shihezi University Xinjiang China
Jia Li - Ministry of Education, Pharmacy Shihezi University Xinjiang China
Shanshan Su - Ministry of Education, Pharmacy Shihezi University Xinjiang China
Wen Chen - Pharmacy Shihezi University Xinjiang China

Objective(s): This study aimed to evaluate the protective effects of total flavonoid extract from Coreopsis tinctoria Nutt.(CTF)against myocardial ischemia/reperfusion injury (MIRI) using an isolated Langendorff rat heart model. Materials and Methods: Left ventricular developed pressure (LVDP) and the maximum rate of rise and fall of LV pressure (±dp/dtmax) were recorded. Cardiac injury was assessed by analyzing lactate dehydrogenase (LDH) and creatine kinase (CK) released in the coronary effluent. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels were determined. Myocardial inflammation was assessed by monitoring tumor necrosis factor-alpha (TNF-α), C-reactive protein (CRP), interleukin-۸ (IL-۸), and interleukin-۶ (IL-۶) levels. Myocardial infarct size was estimated. Cell morphology was assessed by ۲,۳,۵-triphenyltetrazolium chloride and hematoxylin and eosin (HE) staining. Cardiomyocyte apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. Results: Pretreatment with CTF significantly increased the heart rate and increased LVDP, as well as SOD and GSH-Px levels. In addition, CTF pretreatment decreased the TUNEL-positive cell ratio, infarct size, and levels of CK, LDH, MDA, TNF-α, CRP, IL-۶, and IL-۸. Conclusion: These results suggest that CTF exerts cardio-protective effects against MIRI via anti-oxidant, anti-inflammatory, and anti-apoptotic activities.

Anti-apoptosis, Anti-oxidant, Anti-inflammatory, Cardio-protective, Coreopsis tinctoria Nutt