Methylation and mRNA expression levels of P۱۵, death-associated protein kinase, and suppressor of cytokine signaling-۱ genes in multiple myeloma

Objective(s): The aim of this study was to investigate the methylation status and mRNA expression levels of P۱۵, death-associated protein kinase (DAPK), and suppressor of cytokine signaling-۱ (SOCS۱) genes in multiple myeloma (MM). Materials and Methods: The bone marrow samples of ۵۴ MM patients were collected and the methylation status of the P۱۵, DAPK, and SOCS۱ gene promoter regions was determined by methylation-specific polymerase chain reaction. Automated sequencing technology was used to sequence the amplified products in order to analyze the base methylation sites. mRNA expression levels were determined using real-time fluorescent quantitative polymerase chain reaction . Results: Among the ۵۴ MM patients, the positive methylation rates of the P۱۵, DAPK, and SOCS۱ genes were ۲۷.۷۸%, ۱۸.۵۲%, and ۱۶.۶۷%, respectively. The methylation results were confirmed by sequencing. The positive methylation rates of the P۱۵, DAPK, and SOCS۱ genes showed no correlation with patient gender, age, typing, staging, and grouping (P>۰.۰۵). There was no significant difference in the mRNA expression levels of the P۱۵, DAPK, and SOCS۱ genes between the MM patient group and the control group (P>۰.۰۵). Conclusions: Aberrant methylation of the P۱۵, DAPK, and SOCS۱ genes exists in MM, and these genes may play certain roles in pathogenesis of MM. There was no significant difference in mRNA expression levels between the methylated group and the non-methylated group, suggesting that these genes are regulated by other mechanisms during their transcription.

Keywords:

DAPK gene , DNA methylation , Multiple Myeloma , P۱۵ gene , SOCS۱ gene