Study of chondrogenic potential of stem cells in co-culture with chondrons

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1395

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مقاله ژورنالی

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English

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شناسه ملی سند علمی:

JR_IJBMS-19-6_009

تاریخ نمایه سازی: 30 مهر 1400

Abstract:

Objective(s): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work presents a co-culture strategy using human articular chondrons and adipose-derived stem cells (ASCs) from infrapatellar fat pad (IPFP) for cartilage tissue production. Materials and Methods: Isolated stem cells were characterized by flowcytometry. Electrospun and polycaprolactone (PCL) scaffolds (۹۰۰ nm fiber diameter) was obtained from Bon Yakhteh (Tehran- Iran) and human infrapatellar fat pad-derived stem cells (IPFP-ASCs) were seeded on them. IPFP- ASCs on scaffolds were co-cultured with articular chondrons using transwell. After ۲۱ day, chondrogenic differentiation of stem cell was evaluated by determining the genes expression of collagen۲, aggrecan and Indian hedgehog using real- time RT-PCR. Results: Genes expression of collagen۲, aggrecan by IPFP-ASCs did not alter significantly in comparison with control group. Howevers, expression of Indian hedgehog decreased significantly compared to control group (P˂ ۰.۰۵). Conclusion: These findings indicate that chondrons obtained from osteoarthritic articular cartilage did not stimulate chondrogenic differentiation of IPFP-ASCs in co-culture.

Authors

ParisaNikpou
Parisa Nikpou

Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

DaryoushMohammad Nejad
Daryoush Mohammad Nejad

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

HajarShafaei
Hajar Shafaei

Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

LeilaRoshangar
Leila Roshangar

Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

NasserSamadi
Nasser Samadi

Department of Biochemistry and Laboratory Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

Amir MohammadNavali
Amir Mohammad Navali

Department of Orthopedy, Tabriz University of Medical Sciences, Tabriz, Iran

Ali RezaSadegpour
Ali Reza Sadegpour

Department of Orthopedy, Tabriz University of Medical Sciences, Tabriz, Iran

DariushShanehbandi
Dariush Shanehbandi

Department of Immunolog, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

JafarSoleimani Rad
Jafar Soleimani Rad

Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

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