The Impact of Gender on the Inflammatory Parameters and Angiogenesis in the Rat Air Pouch Model of Inflammation
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 12، Issue: 2
Publish Year: 1388
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-12-2_003
تاریخ نمایه سازی: 30 مهر 1400
Abstract:
Objective(s) Air pouch is a well-established inflammatory model in which fluid extravasations; leukocyte migration, angiogenesis and other parameters involved in the inflammatory response can be measured. In this study, the influence of gender on inflammatory parameters has been examined in the air pouch model. Materials and Methods To induce air pouch, adult male and female Wistar rats were anesthetized, then ۲۰ ml and ۱۰ ml of sterile air were injected subcutaneously on the back on day ۰ and day ۳, respectively. On day ۶, inflammation was induced by injection of ۱ ml of carrageenan ۱% into pouches. After ۶ and ۷۲ hr, the rats were sacrificed, pouch fluid was collected in order to determine exudates volume and the accumulated cells were counted using a hemocytometer. Pouches were dissected out and weighed. Angiogenesis of granulomatous tissue was assayed using hemoglobin kit. Results Analysis of our data demonstrated a sexually dimorphic pattern in inflammation parameters both in acute and chronic forms (P<۰.۰۵). The value of angiogenesis in the air pouch model in male rat was higher than that female rats (P<۰.۰۰۱). Conclusion The degree of inflammation and angiogenesis induced in Wistar rat air pouch model is gender-dependent, suggesting that gender may be a key consideration in the design of inflammation experiments.
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Authors
Tahereh Eteraf Oskouei
School of Health and Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
Nasrin Maleki-dizaji
Department of Pharmacology and Toxicology, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Moslem Najafi
Department of Pharmacology and Toxicology, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
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