Effect of WR-۱۰۶۵ on ۶-hydroxydopamine-induced catalepsy and IL-۶ level in rats

Objective(s): Neuroinflammation and oxidative stress play a key role in pathogenesis of Parkinson’s disease (PD). In the present study we investigated the effect of reactive oxygen species (ROS) scavenger WR-۱۰۶۵ on catalepsy and cerebrospinal fluid (CSF) level of interleukin ۶(IL-۶) and striatum superoxide dismutase (SOD) activity  in ۶-hydroxydopamine (۶-OHDA) induced experimental model of PD. Materials and Methods: Seventy two male Wistar rats were divided into ۹ equal groups and ۶-OHDA (۸ μg/۲ μl/rat) was infused unilaterally into substantia nigra pars copmacta (SNc) to induce PD. Catalepsy was measured by standard bar test, CSF level of IL-۶ was assessed by enzyme-linked immunosorbent assay (ELISA) method and SOD activity measured by spectrophotometric method. In pre-treatment groups WR-۱۰۶۵ (۲۰, ۴۰ and ۸۰ μg/۲ μl/rat/day, for ۳ days) was infused into the SNc before ۶-OHDA administration and ۲۱ days later, as a recovery period, behavioral and molecular assay tests were done. Results: Our results showed that pre-treatment with WR-۱۰۶۵ improved (P<۰.۰۰۱) ۶-OHDA-induced catalepsy in a dose dependent manner. In ۶-OHDA-lesioned animals SOD activity in SNc and CSF level of IL-۶ was decreased markedly (P<۰.۰۰۱) when compared with non-lesioned group, while pre-treatment with WR-۱۰۶۵(P<۰.۰۰۱) restored their levels up to the normal range. Conclusion: Our study indicated that pre-treatment with WR-۱۰۶۵ could modulate catalepsy and IL-۶ level in ۶-OHDA-lesioned rats. Also WR۱۰۶۵ could increase SOD activity up to normal range. It can be regarded as an anti-oxidative drug in prevention or adjunctive therapy of PD.

Keywords:

Catalepsy , IL-۶ , Rat , SOD , WR-۱۰۶۵