Tracheal overexpression of IL-۱β, IRAK-۱ and TRAF-۶ mRNA in obese-asthmatic male Wistar rats

Objective(s): Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-۱β, IRAK-۱ and TRAF-۶ mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). Materials and Methods:Twenty male Wistar rats divided to four groups, included, control group with normal diet (C+ND), OVA-sensitized group with normal diet (S+ND), control group with high-fat diet (C+HFD), and OVA-sensitized group with high-fat diet (S+HFD). All animals fed for ۸ weeks with standard pelts or high-fat diet, and then were sensitized and challenged with OVA or saline for another ۴ weeks with designed regimens. At the end of study, trachea isolated and examined for expression levels of IL-۱β, IRAK-۱ and TRAF-۶ mRNA with RT-PCR method. Results:Diet-induced obesity groups developed increased weight, obesity indexes and lipid profiles (P<۰.۰۵ to P<۰.۰۰۱). The expression levels of IL-۱β mRNA in OVA-sensitization groups (S+ND and S+HFD) showed a significantly increased when compared with control group. Also in S+HFD group, expression level of TRAF-۶ mRNA was higher than other groups (P<۰.۰۰۱). IRAK-۱ expression level was high in S+HFD compared with control group.IL-۱β and TRAF-۶ mRNA correlated positively with obesity indexes. Conclusion:The results showed that DIO causes overexpression of IL-۱β, IRAK-۱ and TRAF-۶ mRNA in an experimental model of asthma. Our results suggested that in obese-asthmatic conditions locally production and activation of pro-inflammatory agents can be increased. These findings showed that possible mechanism for obesity-asthma relationships.

Keywords:

Asthma , IL-۱β , IRAK-۱ , Obesity , Tracheal , TRAF-۶