Mucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosa

Objective(s): This study was performed to investigate the effects of mucosal acidification on mRNA expression and protein synthesis of cystathionine gamma lyase (CSE), cystathionine beta synthase (CBS), and mucosal release of H۲S in gastric mucosa in rats. Materials and Methods:Thirty-two rats were randomly assigned into ۴ groups (۸ in each), including: the control group, HCl (۱۰ mM) treated group, HCl (۱۰۰ mM) treated group, and one group to study the effect of Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME). Anesthetized rats underwent tracheostomy and midline laparotomy. Ninety min after the instillation of neutral or acidic solutions, animals were sacrificed and the gastric mucosa was collected to measure the H۲S concentration by ELISA method and to quantify mRNA expression of CSE and CBS by quantitative real-time PCR (qRT-PCR). Protein synthesis was also detected by Western blot method. Results:Mucosal acidification with ۱۰ and ۱۰۰ HCl, significantly increased mucosal levels of H۲S (P<۰.۰۱ and P<۰.۰۰۱) and mRNA (P<۰.۰۱ and P<۰.۰۰۱) and protein expressions of CSE (P<۰.۰۱ and P<۰.۰۰۱). L-NAME treatment reversed H۲S release to control level. Conclusion:Our findings indicated that mucosal acidification with HCl increased mucosal release of H۲S through upregulation of CSE gene and its protein expression. This effect is mainly mediated through the involvement of nitric oxide.

Keywords:

Cystathionine gamma lyase H۲S , Mucosal acidification , Rat