A New Continuous Flow Injection Analysis Method Coupled with NAG-ADF-۳۰۰-۲ Analyzer for Promethazine-HCl by Cadmium Iodide as a Precipitating Reagent

A highly accurate, simple, sensitive turbidimetric method was introduced for the determination of promethazine-HCl using a homemade NAG-ADF-۳۰۰-۲ system with continuous flow injection analysis system. This method is based on reaction between promethazine-HCl and cadmium iodide (CdI۲) in the presence of ammonium acetate to obtain a white precipitate for the ion-pair complex. Turbidity was measured via the attenuation of incident light by precipitated at ۰-۱۸۰°. Optimum parameters were studied to increase the sensitivity for newly developed methodology of promethazine-HCl- cadmium iodide system. The linear dynamic range for the instrument response vs. concentration of promethazine-HCL- was ۰.۲۵-۲۵ mmol. L-۱ for cell A and ۰.۱-۲۰ mmol.L-۱  for cell B, and limit of quantitative (S/N=۱۰) ۳۶۰.۵۵۷۴ µg /sample for cell A and ۲۷۵.۵۸۳۰  µg /sample for cell B respectively with correlation coefficient (r) ۰.۹۹۶۱ for cell no. ۱ (cell A) and ۰.۹۹۶۶ for cell no.۲ (cell  B); RSD % was lower than ۰.۶%. A comparison was made between the newly developed method with UV-Spectrophotometric and turbidimetric method as a classical method by using the standard addition method via the use of t-test at ۹۵% confidence level. In addition, F-test was utilized to predict which of the methods was more precise than other method. The comparison of data revealed that long distance chasing photometer (NAG-ADF-۳۰۰-۲) is the choice with excellent extended detection, wide application and more sensitivity.  

Keywords:

Promethazine-HCL , NAG-ADF-۳۰۰-۲ , continuous flow injection analysis , Cadmium iodide