Publisher of Iranian Journals and Conference Proceedings

Please waite ..
Publisher of Iranian Journals and Conference Proceedings
Login |Register |Help |عضویت کتابخانه ها

Leukemia- Derived Exosomes Activate Migration and Tumor-Associated Genes in Astrocytes Isolated from Human Brain Tissue

مجله بین المللی کودکان، دوره: 9، شماره: 10
Year: 1400
COI: JR_INJPM-9-10_004
Language: EnglishView: 33
This Paper With 9 Page And PDF Format Ready To Download

Buy and Download

با استفاده از پرداخت اینترنتی بسیار سریع و ساده می توانید اصل این Paper را که دارای 9 صفحه است به صورت فایل PDF در اختیار داشته باشید.
آدرس ایمیل خود را در کادر زیر وارد نمایید:


Parisa Zamani Esmati - Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Javad Baharara - Research Center for Animal Development and Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Sajad Sahab Negah - Neuroscience research center, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran
Khadijeh Nejad Shahrokhabadi - Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.


Background: In children with lymphoblastic leukemia (ALL), cancerous metastasis to the central nervous system (CNS) is common, but there is insufficient information on this metastasis and cancer progression process. Further, the role of exosomes on cancer growth and metastasis has been the attention of many studies. Because the astrocytes involve in the blood-brain barrier and stay in contact with peripheral blood , we investigated the effect of exosomes derived Nalm۶ cell line on astrocytes from human brain fetal tissue.    Methods: In this in vitro study, exosomes were isolated from the supernatant of Nalm۶ cell line by centrifugation and identified by DLS, AFM and FE-SEM methods. Astrocytes were isolated from human fetal brain tissue cells and expanded and identified by GFAP antibody with immunocytochemistry. Astrocytes were treated with different concentrations of exosomes (i.e., ۵, ۱۰, ۳۰, and ۵۰ µg/ml),. Proliferation and migration were assessed with Trypan blue staining and scratch methods, respectively. To define tumorogensis changes in astrocytes treated by exosomes, the expression of MMP۹, P۵۳, and Cox۲ genes were investigated by Real-Time PCR.Findings: According to the DLS results, the size of exosomes was about ۴۳ nm. AFM and FE-SEM imaging indicated these exosomes have a spherical shape. Our results showed that the proliferation of astrocytes significantly increased when treated with ۵۰ µg/ml of exosomes.Therefore, the concentration of ۵۰ µg/ml was considered for further experiments. Astrocyte migration was significantly increased compared to the control group. Finally, our results showed tumorogenesis changes in astrocytes by increasing the expression of MMP۹ and Cox۲ and decreasing the expression of p۵۳ at mRNA level.Conclusion: Based on our in vitro study, exosomes derived from ALL as a peripheral tumor can change the behavior of astrocytes as a target for metastasis. Further investigations on this topic are warranted to shed light on metastatic mechanisms.


Paper COI Code

This Paper COI Code is JR_INJPM-9-10_004. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

How to Cite to This Paper:

If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Zamani Esmati, Parisa and Baharara, Javad and Sahab Negah, Sajad and Nejad Shahrokhabadi, Khadijeh,1400,Leukemia- Derived Exosomes Activate Migration and Tumor-Associated Genes in Astrocytes Isolated from Human Brain Tissue,

Research Info Management

Certificate | Report | من نویسنده این مقاله هستم

اطلاعات استنادی این Paper را به نرم افزارهای مدیریت اطلاعات علمی و استنادی ارسال نمایید و در تحقیقات خود از آن استفاده نمایید.


The specifications of the publisher center of this Paper are as follows:
Type of center: Azad University
Paper count: 10,269
In the scientometrics section of CIVILICA, you can see the scientific ranking of the Iranian academic and research centers based on the statistics of indexed articles.

Share this page

More information about COI

COI stands for "CIVILICA Object Identifier". COI is the unique code assigned to articles of Iranian conferences and journals when indexing on the CIVILICA citation database.

The COI is the national code of documents indexed in CIVILICA and is a unique and permanent code. it can always be cited and tracked and assumed as registration confirmation ID.