Kangok Cho - Institute of Sports Health Science, Sunmoon University, Asan city, Chung Nam, ۳۸۰-۷۰۱, S. Korea
Youngju Song - Institute of Sports Health Science, Sunmoon University, Asan city, Chung Nam, ۳۸۰-۷۰۱, S. Korea
Daekeun Kwon - Institute of Sports Health Science, Sunmoon University, Asan city, Chung Nam, ۳۸۰-۷۰۱, S. Korea

Objective(s):This study examined whether conjugated linoleic acid (CLA) supplementation affects insulin sensitivity and peroxisome proliferator-activated receptor gamma (PPAR-γ) and glucose transporter type ۴ (GLUT-۴) protein expressions in the skeletal muscles of rats during endurance exercise. Materials and Methods:Sprague-Dawley male rats were randomly divided into HS (high-fat diet (HFD) sedentary group, n = ۸), CS (۱.۰% CLA supplemented HFD sedentary group, n = ۸), and CE (۱.۰% CLA supplemented HFD exercise group, n = ۸). The rats in the CE swam for ۶۰ min a day, ۵ days a week for ۸ weeks. Results:The serum glucose and insulin contents and homeostasis model assessment of insulin resistance (HOMA-IR) value of the CS and CE were significantly decreased compared to those of the HS. The PPAR-γ protein expressions in the soleus muscle (SOM) and extensor digitorum longus muscle (EDL) were significantly higher in the CE than in the HS. In addition, the PPAR-γ protein expression in the SOM of the CS was significantly higher than that in the HS. On the other hand, the GLUT-۴ protein expression of the SOM in the CE was significantly higher compared to that in the HS. However, there was no significant difference in GLUT-۴ protein expression in the EDL among the groups. Conclusion:CLA supplementation with/without endurance exercise has role in improvement of insulin sensitivity. Moreover, when CLA supplementation was accompanied by endurance exercise, the PPAR-γ protein expression in SOM and EDL and the GLUT-۴ protein expression in SOM were enhanced compared with the control group.

Conjugated linoleic acid, Endurance exercise Insulin, PPAR-γ, GLUT-۴