Laminin matrix promotes hepatogenic terminal differentiation of human bone marrow mesenchymal stem cells

Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJBMS-19-1_005

تاریخ نمایه سازی: 3 آبان 1400

Abstract:

Objective(s):The application of stem cells holds great promises in cell transplants. Considering the lack of optimal in vitro model for hepatogenic differentiation, this study was designed to examine the effects of laminin matrix on the improvement of in vitro differentiation of human bone marrow mesenchymal stem cells (hBM-MSC) into the more functional hepatocyte-like cells. Materials and Methods:Characterization of the hBM-MSCs was performed by immunophenotyping and their differentiation into the mesenchymal-derived lineage. Then, cells were seeded on the laminin-coated or tissue culture polystyrene (TCPS). The differentiation was carried out during two steps. Afterward, the expression of hepatocyte markers such as AFP, ALB, CK-۱۸, and CK-۱۹ as well as the expression of C-MET, the secretion of urea, and the activity of CYP۳A۴ enzyme were determined. Moreover, the cytoplasmic glycogen storage was examined by periodic acid–Schiff (PAS) staining. Results:The results demonstrated that the culture of hBM-MSC on laminin considerably improved hepatogenic differentiation compared to TCP group. A significant elevated level of urea biosynthesis and CYP۳A۴ enzyme activity was observed in the media of the laminin-coated differentiated cells (P<۰.۰۵). Furthermore higher expressions of both AFP and ALB were determined in cells differentiated on laminin matrix. Glycogen accumulation was not detected in the undifferentiated hBM-MSCs, however, both differentiated cells in laminin and TCPS groups demonstrated the intracellular glycogen accumulation on day ۲۱ of hepatogenic differentiation. Conclusion:Taken together, these findings may indicate that laminin matrix can improve terminal differentiation of hepatocyte-like cells from hBM-MSCs. Thus, laminin might be considered as a suitable coating in hepatic tissue engineering designs.

Authors

Zahra Khalaj

Animal and Marine Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Abbas Sahebghadam Lotfi

Animal and Marine Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Maryam Kabir-Salmani

Biomaterials and Tissue Engineering Department, Stem Cell Division, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

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  • ۲.O'Leary JG, Lepe R, Davis GL. Indications for liver transplantation. ...
  • ۳.Kosmacheva S, Seviaryn I, Goncharova N, Petyovka N, Potapnev M. ...
  • ۴.Gómez‐Lechón MJ, Jover R, Donato T, Ponsoda X, Rodriguez C, ...
  • ۵.Vestentoft PS. Development and molecular composition of the hepatic progenitor ...
  • ۶.Spradling A, Drummond-Barbosa D, Kai T. Stem cells find their ...
  • ۷.Fuchs E, Tumbar T, Guasch G. Socializing with the neighbors: ...
  • ۸.Scadden DT. The stem-cell niche as an entity of action. ...
  • ۹.Schaffner F, Poper H. Capillarization of hepatic sinusoids in man. ...
  • Hahn E, Wick G, Pencev D, Timpl R. Distribution of ...
  • He H, Liu X, Peng L, Gao Z, Ye Y, ...
  • Terada T, Nakanuma Y. Expression of tenascin, type IV collagen ...
  • Martinez-Hernandez A, Delgado FM, Amenta P. The extracellular matrix in ...
  • Wewer UM, Albrechtsen R. Carcinoma-associated perisinusoidal laminin may signal tumour ...
  • Quondamatteo F, Scharif K, Herken R. Changes in laminin immunoreactivity ...
  • Friedman SL, Roll FJ, Boyles J, Bissell DM. Hepatic lipocytes: ...
  • Knittel T, Janneck T, Muller L, Fellmer P, Ramadori G. ...
  • Nikolova G, Strilic B, Lammert E. The vascular niche and ...
  • Tanentzapf G, Devenport D, Godt D, Brown NH. Integrin-dependent anchoring ...
  • Tate MC, Garcı́a AJ, Keselowsky BG, Schumm MA, Archer DR, ...
  • Hayashi Y, Furue MK, Okamoto T, Ohnuma K, Myoishi Y, ...
  • Kazemnejad S, Allameh A, Soleimani M, Gharehbaghian A, Mohammadi Y, ...
  • Ayatollahi M, Soleimani M, Tabei SZ, Salmani MK. Hepatogenic differentiation ...
  • Kallis YN, Robson AJ, Fallowfield JA, Thomas HC, Alison MR, ...
  • Takayama K, Nagamoto Y, Mimura N, Tashiro K, Sakurai F, ...
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