Sepideh Tarbali - Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
Shiva Khezri - Department of Biology, Faculty of Science, Urmia University, Urmia, Iran

Objective(s):Multiple sclerosis (MS) is a demyelinating disease. The prevalence of MS is highest where environmental supplies of vitamin D are low. Cognitive deficits have been observed in patients with MS. Oxidative damage may contribute to the formation of MS lesions. Considering the involvement of hippocampus in MS, an attempt is made in this study to investigate the effects of vitamin D۳ on behavioral process and the oxidative status in the dorsal hippocampus (CA۱ area) following the induction of experimental demyelination in rats. Materials and Methods: Animals were divided into six groups. Control group: animals received no surgery and treatment; saline group: animals received normal saline; sham group: animals received ۱۵۰ μl sesame oil IP; vitamin D۳ group: animals received ۵ μg/kg vitamin D۳ IP; lysophosphatidyl choline (LPC) group (toxic demyelination’s model): animals received LPC by stereotaxic intra-hippocampal injection of ۲ μl LPC in CA۱ area; Vitamin D۳- treated group: animals were treated with vitamin D۳ at doses of ۵ μg/kg IP for ۷ and ۲۱ days post lesion. The spatial memory, biochemical parameters including catalase (CAT) activities and lipid peroxidation levels were investigated. Results: Animals in LPC group had more deficits in spatial memory than the control group in radial arm maze. Vitamin D۳ significantly improved spatial memory compared to LPC group. Also, results indicated that vitamin D۳ caused a decrease in lipid peroxidation levels and an increase in CAT activities. Conclusion: Current findings suggest that vitamin D۳ may have a protective effect on cognitive deficits and oxidative stress in toxic demyelination’s model.

Demyelination Hippocampus, Multiple Sclerosis, Oxidative stress, Vitamin D۳