Amir Farshchi - School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
Golbarg Ghiasi - School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
Samireh Farshchi - Department of otolaryngology, Amiralam Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Amin Taleb Ghobadi - School of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran

Objective(s) Diabetes mellitus is associated with disturbances of learning and memory and cognitive functioning. Aegle marmelos Corr. from Rutaceae family is widely used in Iranian folk medicine for the treatment of diabetes mellitus. Considering the beneficial antidiabetic and antioxidant potential of A. marmelos, this study was conducted to evaluate the effect of oral administration of A. marmelos on learning and spatial memory in diabetic rats using Morris water maze test. Materials and Methods Considering the beneficial antidiabetic potential of A. marmelos, this study was conducted to evaluate the effect of chronic oral administration of A. marmelos as cognitive enhancer, on learning and spatial memory in diabetic rats using Morris water maze test. Male Wistar rats were randomly divided into normal-control, diabetic-control, and A. marmelos-treated diabetic groups (۱۰۰, ۲۵۰ and ۵۰۰ mg/kg, p.o.). Animals were treated for ۴ weeks by A. marmelos or normal saline. Diabetes was induced by a single dose i.p. injection of streptozotocin (۴۵ mg/kg). In each group of animals, spatial learning and memory parameters were analyzed. Results Clear impairment of spatial learning and memory was observed in diabetic group versus normal-control group. A. marmelos showed dose dependent improvement in spatial learning and memory parameters that swimming time (Escape Latency) in normal-control and A. marmelos-treated diabetic animals rats was  significantly (P< ۰.۰۱) lower than diabetic-control, while swimming speed was significantly (P< ۰.۰۵) higher. Conclusion The study demonstrated that A. marmelos has significant protective affect against diabetes-induced spatial learning and memory deficits. This effect could be attributed to hypoglycemic, hypolipidemic and antioxidant activity of A. marmelos.

Aegle marmelos, Diabetes, Morris Water Maze, Spatial learning and Memory