Effect of Ethanolic Extract of Indigofera tinctoria on Chemically-Induced Seizures and Brain GABA Levels in Albino Rats

Objective(s) Indigofera tinctoria Linn. of Fabaceae family is claimed to be useful to control epileptic seizures in the Indian system of folkore medicine. This study was designed to evaluate I. tinctoria and to verify the claim. Materials and Methods Seizures were induced in male albino rats with pentylenetetrazole (PTZ). The test group animals were administered ethanolic extract of Indigofera tinctoria (EEIT) orally. The time of onset and duration of clonic convulsions were recorded. Maximal electroshock seizures (MES) were induced in animals. The duration of hind limb tonic extension (HLTE) was recorded. GABA levels and GABA transaminase activity in brain were estimated. Results In PTZ model, EEIT significantly (P< ۰.۰۱, P< ۰.۰۰۱) delayed the onset of convulsions and reduced the duration of seizures in a dose dependent manner. A significant (P< ۰.۰۵) reduction in the duration of HLTE at higher doses of EEIT was observed in MES model. Increase in brain GABA levels was observed on treatment with EEIT at ۵۰۰ and ۱۰۰۰ mg/kg doses, suggested that the plant may be acting by facilitating GABAergic transmission. A significant reduction (P< ۰.۰۵) in the activity of brain GABA transaminase was observed at higher doses. No neurotoxic signs were observed with rotarod test, pentobarbital induced sleeping time, locomotor activity and haloperidol-induced catalepsy. Conclusion The ethanolic extract of I. tinctoria was found to be useful to control and treat the variety of seizures.

Keywords:

Clonic seizures , GABA , GABA-T , Hind limb tonic extension , Indigofera tinctoria , MES , Neurotoxic , PTZ