Aloe vera toxic effects: expression of inducible nitric oxide synthase (iNOS) in testis of Wistar rat
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-18-10_004
تاریخ نمایه سازی: 3 آبان 1400
Abstract:
Objective(s): Nitric oxide (NO), a product of inducible nitric oxide synthase (iNOS), contributes in germ cell apoptosis. This study was aimed to evaluate the effects of Aloe vera gel (AVG) on male Wistar rat reproductive organ, serum NO level, and expression of iNOS gene in leydig cells. Materials and Methods:Adult male Wistar rats (n=۳۶) were used for experiments in three groups. The experimental groups were orally administered with the AVG extract solution once-daily as follow: ۱۵۰ mg.kg-۱; group A, ۳۰۰ mg.kg-۱; group B, and only normal saline; group C (control group). They were mated with untreated females and the reproductive and chemical parameters were assessed for each group, including semen quality, serum testosterone, sperm fertility, gonad and body weight, serum NO concentration (by the Griess method), and iNOS gene expression (using RT-PCR). Results: The testes weight, serum testosterone, as well as sperm count and fertility of the AVG treated groups were significantly reduced when compared to the control (P<۰.۰۰۱). Concentration of serum NO was significantly increased (۳۷.۱±۴.۶۳ µM) in the administrated group with higher AVG concentration, compared to the control group (P<۰.۰۰۱; ۱۰.۱۹±۰.۸۷ µM); however, iNOS mRNA expression was increased in the treated animals (P<۰.۰۰۱). Conclusion:iNOS may play a functional role in spermatogenesis via apoptosis, reducing sperm count, but further studies are needed to illustrate the mechanisms by which AVG exerts its negative effects on spermatogenesis and sperm quality.
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Authors
Samira Asgharzade
Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran
Mahmoud Rafieian-kopaei
Medical Plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Amin Mirzaeian
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Somaye Reiisi
Department of Genetics, Faculty of Basic Sciences, University of Shahrekord, Shahrekord, Iran
Loghman Salimzadeh
Medical Plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
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