Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia

Objective(s): Nucleus Raphe Magnus (NRM) that is involved in the regulation of body temperature contains nitric oxide (NO) synthase. Considering the effect of NO on skin blood flow control, in this study, we assessed its thermoregulatory role within the raphe magnus. Materials and Methods: To this end, tail blood flow of male Wistar rats was measured by laser doppler following the induction of hypothermia. Results: Intra-NRM injection of SNP (exogenous NO donor, ۰.۱- ۰.۲ μl, ۰.۲ nM) increased the blood flow. Similarly, unilateral microinjection of glutamate (۰.۱- ۰.۲ μl, ۲.۳ nM) into the nucleus increased the blood flow. This effectof L-glutamate was reduced by prior intra NRM administrationof NO synthase inhibitor NG-methyl-L-arginine or NG-nitro-L-argininemethyl ester (L-NAME, ۰.۱ µl, ۱۰۰ nM). Conclusion: It is concluded that NO modulates the thermoregulatory response of NRM to hypothermia and may interactwith excitatory amino acids in central skin blood flow regulation.

Keywords:

L-NAME , Nitric oxide , Raphe magnus , Sodium nitroprusside