The activity of ozonated olive oil against Leishmania major promastigotes
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 18، Issue: 9
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-18-9_011
تاریخ نمایه سازی: 3 آبان 1400
Abstract:
Objective(s):Cutaneous Leishmaniasis is a common and endemic disease in Khorasan province in North-East of Iran. The pentavalant antimony (Sb V) is the mainstay of treatment that has many side effects and resistance to the drug has been reported. The microbicidal effect of ozone was proven in different microorganisms. Since there is no study in this respect and to achieve a low cost and effective treatment, we decided to evaluate the efficacy of ozone against promastigotes of Leishmania major,in vitro. Materials and Methods: Ozonated olive oil was prepared after production of ozone by bubbling ozone-oxygen gas produced by ozone generator through olive oil until it solidified. Promastigotes of L. major were cultivated in two phasic media. After calculation of the number of promastigotes, they were incubated with ozonated olive oil (۰, ۰.۶۲۶, ۰.۹۳۸, ۱.۲۵, ۲.۵, ۵, ۱۰ mcg/ml) at ۲۸ °c for ۲۴ hr. Parasites survival percentage was evaluated using MTS and microscopic assay, and then compared with Glucantime and non-ozonated olive oil. Results:According to the results, there were significant differences in parasites survival percentage between ozonated olive oil and non-ozonated olive oil, at similar concentrations (P<۰.۰۰۱). Ozonated olive oil was more effective than Glucantime. According to MTS results, Glucantime and ozonated olive oil gel concentrations that are required to inhibit the growth of L. major promastigotes by ۵۰% (IC۵۰), were ۱۶۵ and ۰.۰۰۲ mg/ml, respectively. Conclusion: Ozonated olive oil has in vitro activity against the promastigotes of L. major and this effect is dose dependent.
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Authors
Omid Rajabi
Department of Medicinal Chemistry, Mashhad University of Medical Sciences, Mashhad, Iran
Ameneh Sazgarnia
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Fatemeh Abbasi
Mashhad University of Medical Sciences, Mashhad, Iran
Pouran Layegh
Cutaneous Leishmaniasis Research Center, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
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