Mutation Analysis of TP۵۳ Tumor Suppressor Gene in Colorectal Cancer in Patients from Iran (Kerman Province)

Objective(s) P۵۳ is an important tumor suppressor, which is mutated in later stages of many cancers and leads to resistance to chemotherapy. The aim of this study was to reveal mutations of TP۵۳ in colorectal cancer in Kerman province. Materials and Methods A total of Forty-three colon cancer specimens as paraffin block or fresh tissues, which passed stage IIIA, were selected. Three exons ۵, ۷ and ۸ of P۵۳ were amplified by PCR technique and sequenced directly. Results The results showed two deletions at codon ۱۴۰ and ۱۴۲ in one tumor sample. GAT→AAT mutation at codon ۱۸۴, and CGG→TGG mutation at codon ۲۴۸ were seen in some tumor samples. Some mutations were also observed in middle of intron ۷ in some tumor or normal tissues. Conclusion Some of those patients with mutation in P۵۳ gene had metastasis in other organs. Therefore, genetic test before chemotherapy is helpful for successful treatment.

Keywords:

Colorectal cancer , Point mutation , tp۳۵ tumor suppressor gene