HPV۱۸ E۷ induces the over-transcription of eIF۴E gene in cervical cancer

Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJBMS-18-7_010

تاریخ نمایه سازی: 3 آبان 1400

Abstract:

Objective(s):Eukaryotic translation initiation factor ۴E (eIF۴E) is overexpressed in cervical cancer (CC). However, the molecular mechanisms are unclear. This study aimed to investigate the molecular mechanism of eIF۴E gene overexpression in CC. Materials andMethods:The human papillomavirus (HPV) type ۱۸ and eIF۴E mRNAs were measured following knock down or overexpression of gene by RT-PCR and real-time PCR. Cell counting kit-۸ assay was used to determine the cell proliferation. Flow cytometry was used to analyze the cell cycle and apoptosis. Transwell system was employed to determine the cell migration. Results:Overexpression of gene increased eIF۴E mRNA level by ۲۴.۳% (P<۰.۰۱) in HPV negative C۳۳A cells. Knock down of decreased markedly eIF۴E mRNA by ۷۳% (P<۰.۰۱) in HPV۱۸ positive HeLa cells. Under the state of high expression of E۷, ۱) up-regulation of eIF۴E drastically promoted the cell proliferation, cell cycle and cell migration, and inhibited the cell apoptosis.  ۲) down-regulation of eIF۴E significantly inhibited the cell proliferation, cell cycle and the ability of cell  migration,  and  also  promoted  the  apoptosis  of  cervical  cancer  cells. Conclusion: HPV induced eIF۴E gene over transcription which might be a new marker for CC. The finding broadens the understanding of the CC carcinogenesis.

Authors

Tianyun Pang

Obstetrics and Gynecology Department of the first Affiliated Hospital of Guangdong Medical College, No. ۵۷ Avenue of the people, Zhanjiang, Guangdong Province ۵۲۴۰۲۳, PR China

Sen Wang

Cancer Institute of Guangdong Medical College, No. ۱ Xincheng Road, Dongguan, Guangdong Province ۵۲۳۸۰۸, PR China

Min Gao

Cancer Institute of Guangdong Medical College, No. ۱ Xincheng Road, Dongguan, Guangdong Province ۵۲۳۸۰۸, PR China

Haixian Kang

Cancer Institute of Guangdong Medical College, No. ۱ Xincheng Road, Dongguan, Guangdong Province ۵۲۳۸۰۸, PR China

Yi Zhao

Cancer Institute of Guangdong Medical College, No. ۱ Xincheng Road, Dongguan, Guangdong Province ۵۲۳۸۰۸, PR China

Yunhong Yao

Pathology Department of Guangdong Medical College, No. ۱ Xincheng Road, Dongguan, Guangdong Province ۵۲۳۸۰۸, PR China

Xinrong Hu

Cancer Institute of Guangdong Medical College, No. ۱ Xincheng Road, Dongguan, Guangdong Province ۵۲۳۸۰۸, PR China

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