The correlation between telomerase activity and Bax/Bcl-۲ ratio in valproic acid-treated MCF-۷ breast cancer cell line

Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
View: 82

This Paper With 5 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

این Paper در بخشهای موضوعی زیر دسته بندی شده است:

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_IJBMS-18-7_012

تاریخ نمایه سازی: 3 آبان 1400

Abstract:

Objective(s):Valproic acid (VPA), a drug used in the treatment of neurological disorders, has been shown to have cytotoxic effects on cancer cells through different mechanisms. Telomerase, a ribonucleoprotein reverse transcriptase, is responsible for elongation of the telomere and is activated in cancers. A relation between telomerase activity and resistance to apoptosis has been established. This study focused on probable effects of VPA on MCF-۷ cancer cells. In particular, we investigated VPA effects on viability, apoptosis and telomerase activity. Materials and Methods: Cytotoxicity effects of VPA on MCF-۷ cells were determined by neutral red uptake assay. Cells were treated with different concentrations of VPA (۰-۳۲ mM) and telomerase activity and Bax and Bcl-۲ protein levels were determined using TRAP assay (PCR-ELISA) method and ELISA method, respectively. Results: The cytotoxic effects of different concentration of VPA on MCF-۷ cells were observed as a reduction in cell viability and telomerase activity and altered expression of Bcl-۲ family protein levels. The results also showed that there is a significant correlation between reduction of telomerase activity and increase in Bax/Bcl-۲ ratio (P=۰.۰۰۱). Conclusion: Our study demonstrated that cell viability of MCF-۷ cells was decreased after treatment with VPA, probably through a reduction of telomerase activity and an increase in Bax/bcl-۲ ratio. Therefore, it could be concluded that VPA is a potent anti-cancer agent for breast cancer cells through inhibition of telomerase activity and induction of apoptosis.

Authors

Zahra Vafaiyan

Department of Biochemistry and Biophysics, Disorder Metabolic Research Center, Golestan University of Medical Sciences, Gorgan, Iran

Roghaye Gharaei

Department of Biochemistry and Biophysics, Disorder Metabolic Research Center, Golestan University of Medical Sciences, Gorgan, Iran

Jahanbakhsh Asadi

Department of Biochemistry and Biophysics, Disorder Metabolic Research Center, Golestan University of Medical Sciences, Gorgan, Iran

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Jemal A, Bray F, Center MM, Ferlay J, Ward E, ...
  • Xia Q, Sung J, Chowdhury W, Chen C-l, Höti N, ...
  • Ververis K, Hiong A, Karagiannis TC, Licciardi PV. Histone deacetylase ...
  • Rahman R, Osteso-Ibanez T, Hirst RA, Levesley J, Kilday J-P, ...
  • Rao Y, Xiong W, Liu H, Jia C, Zhang H, ...
  • Shay JW, Wright WE. Telomerase therapeutics for cancer: challenges and ...
  • Wai LK. Telomeres, telomerase, and tumorigenesisa review. Med Gen Med ...
  • Massard C, Zermati Y, Pauleau A, Larochette N, Metivier D, ...
  • Buseman C, Wright W, Shay J. Is telomerase a viable ...
  • Woo HJ, Lee SJ, Choi BT, Park Y-M, Choi YH. ...
  • Rubis B, Holysz H, Gladych M, Toton E, Paszel A, Lisiak N, ...
  • Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, ...
  • Byun S-S, Kim FJ, Khandrika L, Kumar B, Koul S, ...
  • Fortunati N, Bertino S, Costantino L, Bosco O, Vercellinatto I, ...
  • Li Y, Yin S, Feng S, Nie D, Xie S, ...
  • Khaw AK, Silasudjana M, Banerjee B, Suzuki M, Baskar R, ...
  • Wu P, Meng L, Wang H, Zhou J, Xu G, ...
  • Meeran SM, Patel SN, Tollefsbol TO. Sulforaphane causes epigenetic repression ...
  • Adams JM, Cory S. Bcl-۲-regulated apoptosis: mechanism and therapeutic potential. ...
  • Tsujimoto Y, Shimizu S. Role of the mitochondrial membrane permeability ...
  • Bokelmann I, Mahlknecht U. Valproic acid sensitizes chronic lymphocytic leukemia ...
  • نمایش کامل مراجع