Seyed Mahmoud Amin Marashi - Department of Microbiology & Immunology, Babol University of Medical Sciences, Babol, Iran
Bahram Nasr Esfahani - Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran
Akbar Tavakoli - Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran
Sharareh Moghim - Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran
Mohammad Reza Pourshafie - Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Mansoor Salehi - Departments of Genetics, Isfahan University of Medical Sciences, Isfahan, Iran

Objective(s) Amongst the various antibiotic resistant elements in Vibrio. cholerae, SXT constin (SXT-C) is important. We were going to design a quick method for determination of antibiotic resistance gene pattern in SXT-C. Materials and Methods Ninety four V. cholerae O۱El Tor isolates were used in this study. Antibiotic susceptibility testing, multiplex PCR amplification of SXT-C containing dfrA۱, sulII, strB and the int in a multiplex PCR were done. Results Results of our study showed that ۹۲ (۹۷.۸%) out of ۹۴ isolates were positive for all above genes. Multiplex PCR results correlated with the antibiotic susceptibility data obtained by using disc diffusion assay. Conclusion Using this multiplex PCR, it would be easily possible to demonstrate the presence of antibiotic resistance in SXT-C which, in turn, allows for a suitable treatment in cholera patients causing reduction in the mortality and morbidity rate of the infected individuals.

antibiotic resistance pattern, Integrase, Multiplex-PCR, SXT constin, Vibrio cholerae