Inhibitory effect of corcin on aggregation of ۱N/۴R human tau protein in vitro
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 18، Issue: 5
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-18-5_009
تاریخ نمایه سازی: 3 آبان 1400
Abstract:
Objective(s):Alzheimer's disease (AD) is the most common age-related neurodegenerative disorder. One of the hallmarks of AD is an abnormal accumulation of fibril forms of tau protein which is known as a microtubule associated protein. In this regard, inhibition of tau aggregation has been documented to be a potent therapeutic approach in AD and tauopathies. Unfortunately, the available synthetic drugs have modest beneficial efficacy with several side effects. Therefore, pipeline drugs from natural sources with anti-aggregation properties can be useful in the prevention and treatment of AD. Among medicinal plants, saffron (Crocus sativus, L.), as a traditional herbal medicine has different pharmacological properties and can be used as treatment for several nervous system impairment including depression and dementia. Crocin as a major constituent of saffron is the glycosylated form of crocetin. Materials and Methods: In this study, we investigated the inhibitory effect of crocin on aggregation of recombinant human tau protein ۱N/۴R isoform using biochemical methods and cell culture. Results: Results revealed that tau protein under the fibrillation condition and in the presence of crocin had enough stability with low tendency for aggregation. Crocin inhibited tau aggregation with IC۵۰ of ۱۰۰ µg/ml. Furthermore, transmission electron microscopy images confirmed that crocin could suppress the formation of tau protein filaments. Conclusion: Inhibitory effect of crocin could be related to its interference with nucleation phase that led to increases in monomer species of tau protein. Based on our results, crocin is recommended as a proper candidate to be used in AD treatment.
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Authors
Ali Mohammadi Karakani
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Gholamhossein Riazi
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Seyed Mahmood Ghaffari
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Shahin Ahmadian
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Farzad Mokhtari
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Mahshad Jalili Firuzi
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Seyedeh Zahra Bathaie
Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran
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