Sulforaphane production and antioxidant enzyme activity in Lepidium draba seedlings in presence of Yeast extract and silver nitrate
Publish place: Ethno-Pharmaceutical Products، Vol: 2، Issue: 1
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_ETPHPR-2-1_001
تاریخ نمایه سازی: 4 آبان 1400
Abstract:
Sulforaphane is produced through a hydrolytic reaction catalyzed by myrosinase from glucoraphanin. It has recently attracted researcher's interest due to its anticancer activities. The present study was conducted to evaluate the effects of various concentrations of yeast extract (YE) and silver ion (Ag+) on sulforaphane production level in Lepidium draba seedlings. Our results revealed that the sulforaphane content is increased in seedlings that treated with the both elicitors, but more significantly by Ag+. All elicitor influences are depend on the elicitor dose and time of elicitation and maximized with YE at ۱ mg/mL for ۸ hours and with Ag+ at ۵۰ µM for ۴ hours treatment. It seems that the promotion of sulforaphane content results in induction of glucoraphanin biosynthesis pathway which in turn is mediated by H۲O۲, as revealed by a significant increase in the activity of H۲O۲ scavenger enzymes. DOR: https://dorl.net/dor/۲۰.۱۰۰۱.۱.۲۳۸۳۳۰۱۷.۲۰۲۱.۲.۱.۱.۱
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Authors
Ali Riahi- madvar
Kerman Graduate University of Advanced Technology
Solmaz Sarhady
Department of Biotechnology, Faculty of Science and Modern Technology, Graduate University of Advanced Technology, Kerman, Iran
Mehdi Mohammadi
Department of Biotechnology, Faculty of Science and Modern Technology, Graduate University of Advanced Technology, Kerman, Iran