Study the effect of salt stress on antioxidant compounds of artichoke under invitro conditions
عنوان مقاله: Study the effect of salt stress on antioxidant compounds of artichoke under invitro conditions
شناسه ملی مقاله: JR_ETPHPR-2-1_007
منتشر شده در در سال 1400
شناسه ملی مقاله: JR_ETPHPR-2-1_007
منتشر شده در در سال 1400
مشخصات نویسندگان مقاله:
Masome Rahimbareshi - Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Azim Ghasemnezhad - Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Mehdi Alizadeh - Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Ahad Yamochi - Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
خلاصه مقاله:
Masome Rahimbareshi - Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Azim Ghasemnezhad - Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Mehdi Alizadeh - Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Ahad Yamochi - Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Artichoke (Cynara scolymus) is one of the oldest herbs and nowadays because of its ingredient is concerned by pharmaceutical industry. Abiotic stresses including salinity, changes the metabolic pathways of cultured cells and leads to the production of secondary metabolites. This study was aimed to determine the effect of salt stress on antioxidant compounds of callus focusing on the phenol, flavonoid, and chlorogenic acid and caffeic acid production under in vitro conditions. The present study was carried out as two separate experiments as direct culture (cultured explant) on MS solid medium with different salinity concentrations (۰, ۵۰, ۱۵۰, ۳۰۰, ۶۰۰, ۱۲۰۰ µM NaCl) and indirect culture (callus culture) in different salin concentrations of ۰, ۵۰, ۱۵۰, ۳۰۰, ۶۰۰, ۱۲۰۰, ۲۰۰۰, ۴۰۰۰, ۶۰۰۰ and ۷۵۰۰۰ µM NaCl. Four weeks after culture the mentioned compounds were measured via distinctive methods. Results showed that, versus to indirect culture radical scavenging percentage, the content of chlorogenic acid and caffeic acid were significantly influenced by salinity and the highest amount of them were recorded in the samples in which treated with ۳۰۰ µM NaCl, control and ۶۰۰ µM NaCl, respectively. In indirect culture no significant difference was observed on other phenolic compounds and antioxidant activity except caffeic acid. DOR: https://dorl.net/dor/۲۰.۱۰۰۱.۱.۲۳۸۳۳۰۱۷.۲۰۲۱.۲.۱.۷.۷
کلمات کلیدی: Artichoke, Callus, Phenol, Flavonoid
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1297164/