An in vivo and in vitro investigation on hepatoprotective effects of Pimpinella anisum seed essential oil and extracts against carbon tetrachloride-induced toxicity

Objective(s):  Protective effects of different extracts and essential oil from Pimpinella anisum           L. seeds were examined against carbon tetrachloride (CCl۴)-induced toxicity. The parameters such as serum transaminases, lactate dehydrogenase activity, hepatic glutathione content, liver lipid peroxidation and histopathological changes of liver were assessed as toxicity markers. In the in vitro model of this study, markers such as cell viability, cellular reduced and oxidized glutathione and lipid peroxidation in HepG۲ cells were evaluated.  Materials and Methods:Human liver cancer cell line HepG۲ and male Sprague-Dawley rats were treated with extracts and essential oil, and markers of hepatotoxicity were investigated. Results: The data revealed that the n-hexane extract, effectively attenuated CCl۴‑induced toxicity in both in vitro and in vivo models in current investigation. Conclusion: As the oxidative stress markers were ameliorated, it might be concluded that anise seed possesses protective effects probably due to its antioxidant constituents.

Keywords:

Anise seed , CCl۴ , Hepatotoxicity , HepG۲ cells , Rat , Oxidative stress