Akram Jamshidzadeh - Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Reza Heidari - Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Gerash Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Gerash, Iran
Mojtaba Razmjou - Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Forouzan Karimi - Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Mahmood Reza Moein - Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Omid Farshad - Shiraz University of Medical Sciences, International Branch, Shiraz, Iran
Amin Reza Akbarizadeh - Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Mohammad Reza Houshangi Shayesteh - Shiraz University of Medical Sciences, International Branch, Shiraz, Iran

Objective(s):  Protective effects of different extracts and essential oil from Pimpinella anisum           L. seeds were examined against carbon tetrachloride (CCl۴)-induced toxicity. The parameters such as serum transaminases, lactate dehydrogenase activity, hepatic glutathione content, liver lipid peroxidation and histopathological changes of liver were assessed as toxicity markers. In the in vitro model of this study, markers such as cell viability, cellular reduced and oxidized glutathione and lipid peroxidation in HepG۲ cells were evaluated.  Materials and Methods:Human liver cancer cell line HepG۲ and male Sprague-Dawley rats were treated with extracts and essential oil, and markers of hepatotoxicity were investigated. Results: The data revealed that the n-hexane extract, effectively attenuated CCl۴‑induced toxicity in both in vitro and in vivo models in current investigation. Conclusion: As the oxidative stress markers were ameliorated, it might be concluded that anise seed possesses protective effects probably due to its antioxidant constituents.

Anise seed, CCl۴, Hepatotoxicity, HepG۲ cells, Rat, Oxidative stress