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Title

UV mutagenesis for the overproduction of xylanase from Bacillus mojavensis PTCC ۱۷۲۳ and optimization of the production condition

مجله علوم پایه پزشکی ایران، دوره: 17، شماره: 11
Year: 1393
COI: JR_IJBMS-17-11_004
Language: EnglishView: 46
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Authors

Shokoofeh Ghazi - Department of Microbiology, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
Abbas Akhavan Sepahy - Department of Microbiology, College of Basic Science, Islamic Azad University, Tehran North Branch, Tehran, Iran
Mehrdad Azin - Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran
Khosro Khaje - Department of Biotechnology, College of Basic Science, Tarbiyat Modares University, Tehran, Iran
Ramazanali Khavarinejad - Department of Microbiology, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract:

Objective(s):[p۱]  This study highlights xylanase overproduction from Bacillus mojavensis via UV mutagenesis and optimization of the production process. Materials and Methods:Bacillus mojavenis PTCC ۱۷۲۳ underwent UV radiation. Mutants’ primary screening was based on the enhanced Hollow Zone Diameter/ Colony Diameter Ration (H/C ratios) of the colonies in comparison with the wild strain on Xylan agar medium. Secondly, enzyme production of mutants was compared with parental strain. Optimization process using lignocellulolytic [AGA۲] wastes was designed with Minitab software for the best overproducer mutant. Results: H/C ratio of ۳.۱ was measured in mutant number ۱۷ in comparison with the H/C ratio of the parental strain equal to ۱.۶. Selected mutant produced ۳۳۰.۵۶ IU/ml xylanase. It was ۳.۴۵ times more enzyme than the wild strain with ۹۵.۷۳ IU/ml xylanase. Optimization resulted ۵۷۵ IU/ml xylanase, with wheat bran as the best carbon source, corn steep liquor as the best nitrogen source accompanied with natural bakery yeast powder, in a medium with pH ۷, after ۴۸ hr incubation at ۳۷°C, and the shaking rate of ۲۳۰ rpm. Optimum xylanase activity was assayed at pH ۷ and ۴۰°C. Enzyme stability pattern shows it retains ۶۲% of its initial activity at pH ۹ after ۳ hr. It also maintains up to ۶۶% and ۵۹% of its initial activity after ۱ hr of pre-incubation at ۷۰°C and ۸۰°C. Conclusion: Mutation and optimization caused ۵.۹ times more enzyme yield by mutant strain. Also this enzyme can be categorized as an alkali-tolerant and thermo-stable xylanase.

Keywords:

Paper COI Code

This Paper COI Code is JR_IJBMS-17-11_004. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1297744/

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Ghazi, Shokoofeh and Akhavan Sepahy, Abbas and Azin, Mehrdad and Khaje, Khosro and Khavarinejad, Ramazanali,1393,UV mutagenesis for the overproduction of xylanase from Bacillus mojavensis PTCC ۱۷۲۳ and optimization of the production condition,https://civilica.com/doc/1297744

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