Protection of rats from thioacetamide-induced hepatic fibrosis by the extracts of a traditional Uighur medicine Cichorium glandulosum

Objective(s):To clarify the protective effects of Cichorium glandulosum (CG) extracts on thioacetamide (TAA)-induced rat hepatic fibrosis. Materials and Methods: The dry roots of CG were smashed and percolated with ۹۵% ethanol, and the residual was prepared into petroleum ether extract (CG-V), ethyl acetate extract (CG-VI) and n-butyl alcohol extract (CG-VII). Thirty-six Wistar rats were randomly divided into a normal group, a model group, a CG-V group (۱۵ mg/kg), a CG-VI group (۳ mg/kg), a CG-VII group (۶ mg/kg) and a positive drug group (silibinin capsule, ۸ mg/kg). Organ indices and serum levels of glutamic-oxaloacetic and glutamic-pyruvic transaminases of intragastrically administered rats were obtained. Expressions of FN, Smad۳, IGFBPrP۱ and TGF-β۱ genes were detected by Western Blot and immunohistochemical assays. Apoptosis was examined by TUNEL assay. Results: Hepatic fibrosis of treatment groups was evidently mitigated. Expressions of FN, Smad۳ and TGF-β۱ in administration groups were higher than those in normal group, and moreover were significantly higher in CG-V and CG-VII groups than those of model group. Apoptotic index of model group was significantly higher than that of normal group, but indices of CG-V and CG-VII groups were significantly lower than that of model group. Significantly more FN, Smad۳ and IGFBPrP۱ were expressed in treatment groups than those in normal group. Conclusion: CG extracts may function by altering TGF-β/Smads signal transduction pathway.

Keywords:

Apoptosis , Cichorium glandulosum , Hepatic fibrosis