Quantitative Analysis of GABAA Gamma Receptor Subunits in the Developing Embryonic Chick Forebrain

Objective(s) In this study we investigated the expression of GABAA receptor subunits during brain development. These receptors may change in the embryonic chick forebrain. Materials and Methodes The expression levels of four types of GABAA receptor gamma subunits (γ۱, γ۲, γ۳ and γ۴) were quantified in the embryonic chick forebrain at ۳۲ hr, ۳, ۷, ۱۴, and ۲۰ days of incubation and day one after hatching. The expression level of mRNA in the forebrain of embryonic chicken was measured using real-time RT-PCR. Results The expression level of each subunit increased gradually with development and reached a plateau on ۲۰th day of embryonic development. A reduction was observed on day one after hatching in all gamma subunits. Conclusion This may explain the different physiological and pharmacological function of GABA receptor gamma subunits before and after hatching.

Keywords:

Developmental expression , Embryonic forebrain , GABAA Receptor subunit , Real-time RT-PCR