Preparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 17، Issue: 9
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-17-9_015
تاریخ نمایه سازی: 4 آبان 1400
Abstract:
Objective(s): The purpose of this study was preparation and evaluation of PLGA nanospheres containing the influenza virus and different adjuvants, Quillaja saponin (QS) and CpG-ODN. Materials and Methods: Nanospheres were prepared using the double emulsion-solvent evaporation method. The morphological and physicochemical properties were studied by scanning electron microscopy (SEM), determination of zeta potential, encapsulation efficiency and release profile. Results: The particle size of formulations was less than ۱۰۰۰ nm, except for formulations containing antigen. The results were confirmed with SEM images. Encapsulation efficiency of antigen, QS and CpG ODN were ۸۰%, ۶۲% and ۳۱%, respectively. The zeta potential of nanospheres was about -۳۰ mV. The burst release was observed for all encapsulates and reached to about ۴۸%, ۴۴% and ۳۵% within ۹۰ min for antigen, CpG-ODN and Qs content, respectively. Conclusion: The formulations showed proper physicochemical properties. These nanospheres have good potential to be used as delivery systems/adjuvants for immunization against influenza.
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Authors
Maryam Mohajer
Student Research Committee, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Bahman Khameneh
Department of Food and Drug Control, Students Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
Mohsen Tafaghodi
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
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