Diagnostic Value of Lectins in Differentiation of Molar Placentas

Publish Year: 1391
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJBMS-15-6_004

تاریخ نمایه سازی: 4 آبان 1400

Abstract:

Objective(s)Distinction of hydatidiform moles from non-molar specimens and subclassification of hydatidiform moles as complete and partial are important for clinical practice, but diagnosis based solely on histomorphology suffers from poor interobserver reproducibility. Nowadays, pathologists rely on molecular techniques, however these methods are technically difficult, relatively expensive, and time consuming, and cannot be applied in all laboratories. Therefore, a relatively easy, time- and cost-effective ancillary tool, would be helpful. This study aimed to assess the role of lectins in differential diagnosis of molar placentas.Materials and MethodsLectin histochemistry with a panel of HRP-conjugated lectins comprising SBA, DBA, MPA, PNA, VVA, UEA-۱, LTA, GS-І (B۴), and WGA were performed in ۲۰ non-molar (hydropic and non-hydropic spontaneous abortions) and ۲۰ molar (partial and complete moles), formalin-fixed paraffin-embedded tissue samples. On the basis of staining intensity, sections were graded and Kruskal-Wallis non-parametric statistical test was used to compare differences between samples.ResultsThere was a significant difference between the reactivities of LTA and UEA-І with syncytiotrophoblasts of molar and non-molar specimens (P<۰.۰۰۱). These lectins generally showed a moderate reactivity with syncytiotrophoblasts of molar group but did not react with this cell population in non-molar group. Furthermore, WGA showed relatively increased reaction with syncytiotrophoblasts of molar tissues compared with abortions, however, this did not reach to statistical significance (P=۰.۰۷). No major differences were seen in other lectins reactivities between the studied groups.ConclusionThe present study showed that UEA-۱ and LTA lectins may be used as cytochemical probes in differentiating molar from non-molar placentas, but did not differentiate partial moles from complete moles.

Authors

Fatemeh Atabaki Pasdar

Department of Anatomy and Cell Biology, Mashhad University of Medical Science, Mashhad, Iran

Alireza Khooei

Department of Pathology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran

Alireza Fazel

Department of Anatomy and Cell Biology, Mashhad University of Medical Sciences, Mashhad, Iran

Mahmoud Mahmoudi

Immunology Research Centre, BuAli Research Institue, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Reza Nikravesh

Department of Anatomy and Cell Biology, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Khaje Delui

Department of Medical Ethics, Mashhad University of Medical Sciences, Mashhad, Iran

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  • Soper JT, Mutch DG, Schink JC and American College of ...
  • Berkowitz RS, Goldstein DP. Diagnosis and management of primary hydatidiform ...
  • Soper JT. Gestational trophoblastic disease. Obstet Gynecol ۲۰۰۶; ۱۰۸:۱۷۶-۱۸۷ ...
  • Howat AJ, Beck S, Fox H et al. Can Histopathologists ...
  • Fukunaga M,Katabuchi H,Nagasaka T,et al .Interobserver and intraobserver variability in ...
  • Crips H, Burton JL, Stewart R, Wells M. Refining the ...
  • Walker RA. The use of lectins in histopathology. Pathol Res ...
  • Pilobello K, Mahal LK..Lectin microarrays for glycoprotein analysis. Methods Mol ...
  • Fazel AR, Schulte BA, Spicer SS.Glycoconjugate unique to migrating primordial ...
  • Fazel AR, Thompson R.P, Shumida H, Shulte BA Lectin histochemistry ...
  • Ebrahimzadeh Bideskan AR, Hassanzadeh Taheri MM, Nikravesh MR, Fazel AR. ...
  • Nasir E, Lalani M, Bulmer JN, Wells M. Peroxidase-labeled lectin ...
  • Cooper HS. Peanut lectin binding sites in large bowel carcinoma.Laboratory ...
  • Itzkowitz S H, Yuan M, Montgomery C K, Kjeldsen T, ...
  • Silva F G, Nadasdy T, AND Laszik Z. Immunohistochemical and ...
  • Juan FT, Hoshina AM, Mochizuki M. Lectin binding in tissues ...
  • Sgambatia E, Biagiotti R, Marini M and Brizzi E. Lectin ...
  • Bancroft JD, Gamble M. Theory and Practice of histological techniques. ...
  • Lee MC and Damjanov I Lectin histochemistry of human placenta. ...
  • Jones CJP, Dantzer V, Leiser R and Krebs C. Localisation ...
  • Tatsuzuki A, Ezaki T, Makino Y, Matsuda Y, Otha H. ...
  • Goldstein IJ and Poretz RD. Isolation, physicochemical characterization and carbohydrate-binding ...
  • Schulte BA and Spicer SS. Histochemical evaluation of mouse and ...
  • Whyte A. Lectin binding by microvillous membranes and coated-pit regions ...
  • Jones CJP, Dantzer V, Leiser R and Krebs C. Localisation ...
  • Truman P,Ford HC.The brush border of the Human term placenta. ...
  • Lang I, Hartmann M, Blaschitz A, Dohr G, Skofitsch G ...
  • Thrower S, Bulmer JN, Griffin NR and Wells M. Further ...
  • Jeffrey IJM, Mosley SJ, Jones CJP, Stoddart RW.Proteolysis and lectin ...
  • Sgambati E, Marini M, Vichi D, Zappoli G, Parretti E, ...
  • ۳۱.Richter DU, Jeschke U, Makovitzky J, Goletz S, Karsten U, ...
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