Evaluating the Expression of Oct۴ as a Prognostic Tumor Marker in Bladder Cancer

Objective(s)The key transcriptional regulator Oct۴ is one of the self-renewal and differentiation-related factors in cancer stem cells, where it maintains "stemness" state. Cancer stem cells have been identified in a variety of solid malignancies. They are a small population of tumor cells with stem cell characteristics, which are a likely cause of relapse in cancer patients. Due to high incidence, mortality, and recurrence rates of bladder cancer and the necessity of accurate prediction of malignant behavior of the tumors, we evaluated the prognostic value of Oct۴ expression in formalin-fixed paraffin-embedded (FFPE) tissues of bladder cancer.Materials and MethodsIn this study, Oct۴ expression was evaluated in ۵۲ (FFPE) tissues of bladder cancer. RNA extraction from samples of ۳۰ patients from the archive of Labbafi-Nejad Medical Centre in Tehran was performed and Oct۴ expression levels were examined by semi-quantitative RT-PCR. The intracellular distribution of Oct۴ protein was also determined by immunohistochemistry (IHC).ResultsThe results revealed a significant correlation between the expression level of Oct۴ and the tumors’ grade and stage. A mostly cytoplasmic distribution of Oct۴ protein was also confirmed by IHC.ConclusionAll together, our data indicate that the expression level of Oct۴ gene is correlated with the clinical and histopathological prognostic indexes of tumors and thus can be considered as a potential prognostic tumor marker.

Keywords:

Bladder cancer , Cancer Stem cell , Oct۴ , Prognosis