Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats

Objective(s):To know whether Scurrula atropurpurea is able to modulate total plasma nitrate/nitrite levels, decrease endothelial damage, and increase endothelial progenitor cells (EPCs) in hypertensive rats. Materials and Methods: The rats were divided in ۵ groups: control (normotensive) group, Desoxy cortico sterone (DOCA)-salt hypertensive group, and three DOCA-salt hypertensive groups. All ۵ groups received methanolic extract of S. atropurpurea (MESA) at a dosage of ۵۰; ۱۰۰; and ۲۰۰ mg/KgBW. Serum nitric oxide (NO) was assayed by colorimetric. Circulating endothelial cells (CECs) and EPCs were assayed using flow cytometry. Results: The administration of MESA۱۰۰ and MESA۲۰۰ elevated the total plasma nitrate/nitrite levels but cannot reach the level in control group.  MESA۱۰۰ and MESA۲۰۰ also elevated the EPCs number compared with hypertensive group. The administration of MESA significantly (P< ۰.۰۵) decreased the CECs number compared to hypertensive groups. Conclusion: Methanolic extract of S. atropurpurea is able to modulate total plasma nitrate/nitrite levels and diminish endothelial damage via increasing EPCs.

Keywords:

Endothelial damage , Endothelial prgenitor cells , Hypertensive , Nitric oxide