The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 17، Issue: 8
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-17-8_014
تاریخ نمایه سازی: 4 آبان 1400
Abstract:
Objective(s):Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage. Materials and Methods: Isolated rBMMSc were grown in DMEM supplemented with ۱۰% FBS. The cells were exposed to different concentration of extract. After ۲۱ days, Alizarin red staining, alkaline phosphatase assay and RT-PCR were performed. The results were analyzed by ANOVA software and P value Results: Morphological methods revealed that appropriate concentrations of extract increased osteogenic differentiation in a dose-dependent manner. RT-PCR revealed that extract without or with osteogenic medium due to osteopontin expression had a potential role in osteogenesis. Conclusion: Based on our data it concluded that S. cucumber extract stimulated Bone marrow mesenchymal cells to differentiate into osteogenic lineage without existence of osteogenic medium
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Authors
Javad Baharara
Department of Biology & Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Elaheh Amini
Developmental Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Mohammad Amin Kerachian
Department of Medical Genetics & Medical Genetic Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mozhgan Soltani
Department of Biology, Research & Science Tehran Branch, Islamic Azad University, Tehran, Iran
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