Glutathione s-transferase M۱ and T۱ genetic polymorphisms in Iranian patients with glaucoma

Objective(s):Glaucoma is the second leading cause of blindness and it is related to oxidative stress based on numerous studies. Glutathione S-transferases (GSTs) are members of multigenic family, which have important role in cells as an antioxidant. In the present study, we examined the polymorphism of GSTT۱ and GSTM۱ deletion genotypes (T۰M۱, T۱M۰, and T۰M۰) in ۱۰۰ Glaucoma patients (۴۱with primary open angle glaucoma (PCAG), and ۵۹ with primary closed angle glaucoma (POAG)) compared to ۱۰۰ healthy subjects. Materials and Methods: GSTM۱and GSTT۱ polymorphisms were determined by multiplex polymerase chain reaction. Results: GSTM۱ and GSTT۱ null deletions genotypes were determined in ۲۲ (۵۳.۷%) and ۷ (۱۷.۱%) patients with PCAG and ۳۴ (۳۴%) and ۱۵ (۱۵%) in healthy subjects[VAIO۱] . Comparison between patients and healthy subjects regarding GSTM۱ and GSTT۱ genotypes revealed increase of GSTM۱ null deletions genotypes in patients with PCAG (P=۰.۰۳). Conclusion: It was concluded that the increased frequencies of GSTM۱ null in patients with PCAG could be a risk factor for incidence of PCAG in the Iranian population.  

Keywords:

glaucoma , Glutathione s-transferases , Primary closed angle glaucoma , Primary open angle glaucoma