بررسی اثرات افزودن لاکتوباسیلوس ال بوچنری به جیره غذایی گاو شیری آلوده به آفلاتوکسین B۱ بر فراسنجه های تولید گاز در شرایط آزمایشگاهی

Aflatoxin B۱ is produced by Aspergillus falvus and Aspergillus parasiticus which affect rumen motility and feed degradation. The aim of this study was to evaluate the effects of aflatoxin B۱ on rumen fermentation in the presence of Lactobacillus buchneri additive using in vitro gas production technique. Experimental treatments were: basal diet, basal diet with ۰.۵ mg/ml aflatoxin B۱, basal diet with ۱ mg/ml aflatoxin B۱, and aflatoxin B۱ contaminated base diet (۱ mg/ml) with addition levels of Lactobacillus buchneri ۰.۰۱, ۰.۱ and ۰.۲ ng/kg diet, respectively. Gas volume of each bottles were recorded at ۲, ۴, ۶, ۸, ۱۲, ۲۴, ۳۶, ۴۸, ۷۲, ۹۶ and ۱۲۰ hours of incubation. The data were analyzed in a completely randomized design with three replications. According to the results of gas production, aflatoxin B۱ affect the rumen fermentation and reduced gas production volume (۴۲۹ ml) compared to control group (۳۸۲ ml) (P <۰.۰۵). At ۱۲۰ h incubation time, the highest and lowest gas production volume was related to control group  and treatment with ۰.۲ ng L. buchneri (P <۰.۰۵). Addition of Lactobacillus buchneri caused an increase in the estimated parameters include ME, NEL and organic matter digestibility. Also, the L. buchneri addition at levels of ۰.۰۱, ۰.۱ and ۰.۲ ng/kg significantly reduced pH of diet contaminated with aflatoxin B۱ (P<۰.۰۵). According to the results it concluded that, in vitro digestibility increased by L. buchneri and it has ability to inhibit aflatoxin B۱ reverse effect in in vitro rumen fermentation of dairy cattle diet.

Keywords:

آفلاتوکسین B۱ , لاکتوباسیلوس ال بوچنری , تولید گاز