Association of the single nucleotide polymorphism C۱۸۵۸T of the PTPN۲۲ gene with unexplained recurrent pregnancy loss: A case-control study

Background: Lymphoid-tyrosine-phosphatase which is encoded by the protein tyrosine phosphatase non-receptor ۲۲ (PTPN۲۲) gene plays a pivotal role in the regulation of immune responses by dephosphorylating several signaling intermediates of immune cells. Objective: Since a balanced immune response has been shown to be important during pregnancy, the purpose of this research was to compare the frequency of the PTPN۲۲ C۱۸۵۸T polymorphism in women with unexplained recurrent pregnancy loss (URPL) vs. in a control group for the first time. Materials and Methods: Genomic DNA from ۲۰۰ individuals with URPL and ۲۰۰ individuals without URPL (the control group) at the infertility center in Yazd, Iran was isolated using the salting-out method. The PTPN۲۲ C۱۸۵۸T polymorphism of the two groups was analyzed using polymerase chain reaction-restriction fragment length polymorphism. Genotype frequencies in the women with URPL and the fertile control group were compared using the Chi-square test. Results: There were significant differences in the frequency of the PTPN۲۲ ۱۸۵۸T polymorphism in the URPL individuals vs. the healthy controls, i.e. ۳۲.۰% and ۲۱.۵%, respectively (p = ۰.۰۱). Conclusion: Our findings suggest that the PTPN۲۲ ۱۸۵۸T polymorphism could play a role in recurrent pregnancy loss. Therefore, genotyping of the mentioned polymorphism can help clinicians to predict the probable risk of URPL.

Keywords:

Recurrent pregnancy loss , PTPN۲۲ protein , Single nucleotide polymorphism. , سقط مکرر , پروتئین PTPN۲۲ , پلی مورفیسم تک نوکلئوتیدی.