Portulaca oleracea shows no ameliorative potential on ovariectomy-induced hormonal and estrous cycle dysregulation in normal cyclic rats: An experimental study

Background: Portulaca oleracea (PO) is a widely known plant utilized for its medicinal attributes in the treatment of different illnesses. Objective: To investigate the effect of methanolic extract of PO (MEPO) on ovariectomy-induced reproductive toxicity in normal cyclic rats. Materials and Methods: Twenty ۱۰-wk-old normal cyclic rats weighing ۱۱۰-۲۰۰ g were randomly divided into four groups (n = ۵/group). Group A served as the control and received distilled water only. Group B was ovariectomized without treatment, while groups C and D were ovariectomized but treated with ۴۰۰ and ۸۰۰ mg/kg of MEPO, respectively, for ۱۴ days. At the end of the experiment, body weight, serum hormonal levels, and estrous cycles were monitored across the groups. Results: Groups B, C, and D showed estrous cycle dysregulation and specific phase arrest when compared with the control. While a significant decrease in estradiol (p ≤ ۰.۰۰۱) and testosterone levels (p ≤ ۰.۰۰۱) were observed in groups B, C, and D, only groups C and D showed a significant increase in progesterone level when compared with the control (p ≤ ۰.۰۰۱, p = ۰.۰۱, respectively). Conclusion: The administration of ۴۰۰ and ۸۰۰ mg/kg MEPO is ineffective in ameliorating estrous cycle disruption and hormonal changes seen in ovariectomized normal cyclic adult Wistar rats.

Keywords:

Portulaca oleracea , Wistar rat , Ovariectomy , Estrous cycle , Hormones. , Portulacaoleracea , موش ویستار , تخمدان , چرخه استروس , هورمون­ها.