Mohammadreza Rostami - Department of environmental health engineering, school of public health, Tehran university of medical sciences, Tehran, Iran
Azam Ahmadi - Department of Food Sciences and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
Parisa Ahmadi - Department of Food Sciences and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

The aim of this work was to produce a novel degradable film with gelatin / chitosan and resveratrol, and the electrospinning process was applied for the encapsulation of resveratrol in nanofibers from gelatin and chitosan solution. The optimized ratio of gelatin: chitosan: resveratrol was found to be ۲: ۱: ۰.۰۵ (w/v/v) after the evaluation of electrospinning solutions and nanofiber physical properties. The results showed that resveratrol could be efficiently encapsulated in micro-nanofibers (۸۸.۷۲ g/۱۰۰ g). The active layer containing it showed a uniform fiber morphology observed by scanning electron microscope and obtained uniform diameter at ۲۰۰±۴ nm. The tensile strength of nanofibers reached ۱.۸۳ ± ۰.۰۴ MPa. The diameter of nanofibers was enlarged and the hydrophobicity was enhanced, the antibacterial activity and antioxidant activity wereimproved as the addition of resveratrol compared with pure gelatin nanofibers. In particular, nanofibers containing ۵ μL/mL resveratrol showed the highest antioxidant activity (۳۳.۹۹ mg DPPH/g dry weight), radical scavenging activity (۴۴.۸۰%), and significant microbial growth inhibition with CFU of about ۳.۲ log units (E. coli) and ۲.۷ log units (S. aureus) lower than the control. At last, the nanofiber was applied to preserve beef and exhibited antibacterial activity against Listeria monocytogenes during ۱۰-days storage at ۴ °C and ۷-days storage at ۱۲ °C, without impact on the sensory and surface color properties. In conclusion, our results suggested that active layer could be an effective method to encapsulate resveratrol, and the chitosan/gelatin nanofibers was a novel biodegradable packaging material with great antioxidant and antibacterial activity.

Resveratrol, Nanofiber, Electrospinning, Active Packaging