N Montaser - Department of Chemical engineering, Azad university, Science and Research Campus, Poonak sq, Ashrfiesfahani high way, Tehran, Iran. Tel: +۹۸۲۱-۴۴۸۶۹۷۲۴-۲۶, fax: +۹۸۲۱-۴۴۸۰۴۲۷۵
M Ardjmand - Department of Chemical engineering, Azad university, Tehran South Branch, Ahang St, Nabard St, Piroozi Ave, Tehran, Iran. Tel: +۹۸۲۱-۳۳۷۲۲۸۳۱-۵
A Heidari nasab - Department of Chemical engineering, Azad university, Science and Research Campus, Poonak sq, Ashrfi esfahani high way, Tehran, Iran. Tel: +۹۸۲۱-۴۴۸۶۹۷۲۴-۲۶
A Safe kordi - Department of chemical and petroleum engineering, Sharif university of technology, Azadi St, Tehran, Iran. Tel: +۹۸۲۱-۶۶۱۶۵۴۱۸, fax: +۹۸۲۱-۶۶۰۳۶۰۰۵

Azotobacter beijerinckii DSMZ 1041 strain was used for production of polyhydroxybutyrate (PHB) and the optimum glucose concentration, as the carbon source and ammonium chloride, as the nitrogen source were obtained 60gr/lit and 0.5gr/lit respectively. The resulting optimum PHB production was investigated 2.95gr/lit. The obtained yield of PHB with respect to glucose (Yp/s) and PHB productivity (Qp) were 0.202 g/g and 0.123 g/lit.hr, respectively. The response surface methodology (RSM) was used to verify the accuracy of the designed experiments azotobacter beijerinckii microorganism from Deutsch sammlung von microorganismen und zellkulturen(DSMZ) bank is used for production of Poly Hydroxy Butyrate (PHB) with consider optimum condition relative to carbon and nitrogen source at this study. Chloride ammonium as nitrogen source with 0/5, 1 and 2gr/lit concentration and glucose as carbon source with 30, 40, 50 and 60gr/lit concentration is examined until earning best condition of experimental test. increasing of glucose concentration and decreasing of chloride ammonium concentration will be affected as inhibitor culture, therefore boundary limit concentration is determined. The optimized shaking rate, temperature and seed age were obtained 180 rpm, 30◦C and 15 hrs, respectively. FT-RI and NMR is revealed biopolymer corrected at solution.The maximum biopolymer P (3HB) concentration was obtained 2.95g/lit corresponding to 6.5g/lit biomass concentration and 14.6 gr/lit carbon consumption using 60gr/lit glucose and 0.5gr/lit chloride ammonium as media after 24 hours. In this media Yp/s and Qp were 0.202g/g and 0.123g/lit.h respectively.

Biopolymer, Poly-β-Hydroxybutyrate (PHB), Biodegradable, Bioplastic, Batch Fermentation, Polyhydroxyalkanoate