امیرسجاد جعفری - Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
سحر اسدالهی زوج - Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.
مهدی راسخ - Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
داریوش سعادتی - Department of Nutrition and Animal Breeding, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
فائزه فقیهی - Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
مهدی فضلعلی پور - Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran
ابوالفضل لطفی - Damavand Agricultural College, Technical and Vocational University, Tehran, Iran
مجتبی بیک محمدی - North West of Tehran Health Center, Iran University of Medical Sciences, Tehran, Iran
اسداله حسینی چگنی - Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

Ticks are vectors for several important zoonoses including different species of Anaplasma. The present study aims to determine the presence of Anaplasma spp. in hard ticks collected from livestock of South-Khorasan province, Iran. A total of ۶۸۴ livestock were sampled and ۲۶۹ ticks were collected. Two genera and ۶ species of ticks were identified including Rhipicephalus sanguineus, Hyalomma detritium, Hyalomma marginatum, Hyalomma anatolicum, Hyalomma asiaticum, Hyalomma dromedarii and Hyalomma spp. Eleven Hyalomma nymphs and ۳ Rhipicephalus nymphs were also identified. ۱۰۰ Out of ۲۶۹ ticks were chosen for molecular detection. DNA was extracted followed by PCR technique to detect Anaplasma spp. The presence of Anaplasma spp. was confirmed in ۲۰ out of ۱۰۰ tested samples (۲۰%). All positive samples collected from Birjand county were Rhipicephalus sanguineus. Results of the present study showed a relatively high infection rate of Anaplasma in hard ticks in South-Khorasan Province.

Ixodidae, PCR, Hyalomma, Rhipicephalus