Akram Mahna - Department of Medical Physics, faculty of medicine, Tabriz university of medical sciences, Tabriz, Iran
Seyed Mohamad Firoozabadi - Department of Medical Physics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Amir Atashi - Department of medical laboratory sciences, school of allied medical sciences, Shahroud university of Medical sciences, Shahroud, Iran

Introduction: Over the last decades, there has been an increasing trend in using cell phones which are exposing us to Radio-Frequency (RF) and Extremely Low Frequency (ELF) magnetic fields with various known and unknown biological effects. This protective study aimed to investigate the impact of environmental ۲۱۷ Hz (as an ELF) magnetic fields generated by mobile phones on angiogenesis as an essential factor in tumor growth, in vitro and in vivo. Material and Methods: Magnetic fields with amplitudes of ۰.۵, ۶, ۲۲, ۴۴, ۶۵ & ۱۵۹ µT were exposed on Human umbilical vein endothelial cells (HUVECs) and proliferation and viability of cells were measured. ۳D angiogenesis assay was done by culturing HUVEC-covered microbeads in collagen gel and counting the number of sprouting micro-vessels per microbead. The percent of CD۳۱ positive areas in breast tumor tissues of mice was assessed in the in vivo study. Results: Results showed that some of the applied amplitudes could increase proliferation as well as the viability of HUVECs. Furthermore, ۲۲ and ۴۴ µT magnetic fields could significantly increase the angiogenesis of breast tumors in the mouse. Conclusion: There is a promoting effect from ELF magnetic fields generated by cell phones on the angiogenesis of tumors. It will be helpful if we recommend that cancer patients not to be exposed to cell phones.

Magnetic Field, Angiogenesis, Endothelial cells, Mice, Breast Cancer