Prevalence and Antibiotic Resistance of Listeria Monocytogenes in Chicken Meat Retailers in Yazd, Iran

Introduction: Listeria monocytogenes is a pathogen bacteria transmitted by food stuffs. Due to the lack of information about contamination of retail chicken meat to Listeria monocytogenes in Iran, this study aimed to determine the prevalence and antibiotic resistance of this bacterium in retail chicken meat in Yazd, Iran. Materials and Methods: This cross-sectional descriptive study was conducted from January ۲۰۱۸ to June ۲۰۱۸ on ۸۱۱ randomly selected samples from four districts located in Yazd city, Iran. Isolation of Listeria monocytogenes was performed using enrichment and selective culture media as well as biochemical tests. The positive samples were confirmed by PCR assay. Results: In total, ۲۴۷ samples (۳۰.۵%) were infected with at least one of the Listeria spp. Among the ۲۴۷ samples, the isolates were ۶۸ (۲۷%) L. monocytogenes, ۱۵۵ (۶۳%) L. innocua, ۵ (۲%) L. seeligeri, ۱۹ (۸%) L. ivanovii. In current study, the antibiotic resistance of positive samples was also evaluated that especially Listeria monocytogenes were resistant to tetracycline and penicillin. Conclusions: The presence of this pathogenic microorganism in chicken meat can be a health risk, especially for pregnant women, the elderly, and those with immune deficiency. Considering the amount of contamination with L. monocytogenes in chicken and the high mortality rate caused by it, monitoring of the health principles and standards during the production, transportation, and storage as well as training of employees in this industry are necessary.

Keywords:

Listeria Monocytogenes , Chicken Meat , PCR.