Samira Moradzadeh - Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran
Sina Siavash Moghaddam - Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran
Amir Rahimi - Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran
Latifeh Pourakbar - Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
R.Z SAYYED - Department of Microbiology, PSGVP Mandal’s Arts, Science, and Commerce College, Shahada, Maharashtra ۴۲۵۴۰۹, India.

Nigella sativa L. is a nutraceutical herb with extensive pharmaceutical and biological properties. Currently, reducing the application of chemical fertilizers (synthetic fertilizers) causes serious problems toenvironmental pollution and human health. Therefore, the combined effect of urea and biofertilizers was studied on the antioxidants traits of N. sativa L. in a randomized complete block design with ۱۰ treatments and three replications. The treatments included control (no fertilization), U (۱۰۰% chemical fertilizer as urea), Nb (Nitrogen biofertilizer) , Pb (Phosphate biofertilizer) , Kb (Potash biofertilizer) , NPKb ( NPK biofertilizer ), Nb+ ۵۰% U , Pb + ۵۰% U , Kb +۵۰%U and NPKb + ۵۰%U. The analysis of variance revealed that the treatments affected all the traits significantly (P < ۰.۰۱). The highest total DPPH free radical scavenging (۳۳.۴۷% ), Superoxide radical scavenging (۵۹.۵۰%) and chain-breaking activity (۱.۰۲%) were related to the treatment of NPKb + U۵۰%. There is, therefore, a definite need for applying combined fertilizer system as a method to achieve sustainable agriculture. In sum up, the application of NPKb + U۵۰% as bio-chemical fertilizers improved N. sativa L antioxidants attributes.

Synthetic fertilizer, Biofertilizer, Antioxidants, Black cumin.