Hairy root Induction in Nicotiana tabacum L.

Plants produce a large and diverse group of organic compounds called secondary metabolites. Such a compounds synthesized with a low rate in plants and they usually accumulated in differentiated tissues. Tissue culture technique is being considered as an alternative technique for agricultural processes for production of valuable secondary metabolites. Hairy root culture is the most promising system for the production of these valuable compounds. for this, the induction of hairy root in tobacco (Nicotiana tabacum L.) was performed using two Agrobacterium rhizogenesis strains including ۱۵۸۳۴ and R۱۰۰۰. The effect of explant type (leaves and petioles) and bacterial density (OD ۶۰۰nm= ۰.۲, ۰.۴ and ۰.۶) were evaluated based on factorial experiments in a completely randomized design with three replications. The results showed that the highest percentage of transgenics for both strains is related to leaf explants and different densities of bacteria do not have a significant effect on hairy root induction. The present study showed that both the two strains induced hairy roots in tobacco.

Keywords:

Hairy root culture , explant , Agrobacterium rhizogenesis , Transgenic , Nicotiana tabacum L.