Effect of isolated polysaccharide from Rosa canina on the methylation of insulin and Pax۴ in diabetic rats

Publish Year: 1400
نوع سند: مقاله کنفرانسی
زبان: English
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BMPM04_041

تاریخ نمایه سازی: 22 آذر 1400

Abstract:

Introduction: Since DNA methylation plays a causal role in β-cell remodeling and development, thus modulation of this epigenetic mechanism is essential in treatment of diabetes. Therefore, this provoked us to examine the effect of a known anti-diabetic agent, an isolated polysaccharide, on the methylation pattern of Ins-۱ and Pax-۴ in diabetic rats. Materials: Here, a polysaccharide fraction was isolated from Rosa canina and analyzed using NMR, FTIR and MS/MS techniques. Diabetes was established by using intraperitoneal injection of STZ in male Wistar rats. After treatment, pancreas was removed and DNA was extracted and bisulfite treated by a DNA methylation kit. PCR and real-time PCR were used to determine the levels of methylated and/or unmethylated Ins-۱ and Pax-۴ genes.Results: The levels of blood glucose and weight body were normalized in diabetic rats exposed to isolated polysaccharide. The level of unmethylated Ins-۱ was upregulated in diabetic rats which is downregulated in metformin and polysaccharide-treated ones. In diabetic rats, the content of methylated Pax-۴ was increased while it was decreased in polysaccharide-treated group. Interestingly, the methylation pattern of Pax-۴ in metformin group was the same as diabetic ones.Conclusion: Data clearly indicated that polysaccharide can reduce the level of blood glucose by modulating the methylation pattern of Pax-۴ and Ins-۱. This study sheds light on the importance of DNA methylation modulation as a promising therapeutic strategy in diabetes.

Authors

Soraya Sajadimajd

Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.

Gholamreza Bahrami

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.