Bioinformatics study of three key enzymes of phenylpropanoids biosynthesis pathway

Phenylpropanoids are important secondary metabolites that plants produce in the response to biotic and abiotic stimuli. We studied bioinformatic criteria of mRNA of three key enzymes (from medicinal plants (Ocimum basilicum and Withania somnifera) of phenylpropanoids biosynthesis pathway. Cinnamate ۴-hydroxylase (C۴H) mRNA (HM۹۹۰۱۵۰.۱, ۱۵۱۸ nt), ۴-coumaroyl CoA-ligase (۴CL۴) mRNA (KC۵۷۶۸۴۱, ۱۷۰۴ nt) and ۴-Coumarate ۳-hydroxylase (C۳H) mRNA (HM۵۸۵۳۶۹.۱, ۱۷۶۹ nt) encoded proteins consisting of ۵۰۵, ۵۷۶ and ۵۱۱ amino acids, respectively. Three, one, and one conserved region(s) were detected in these polypeptide chains, respectively. mRNA sequences showed high homology with C۴H Ocimum tenuiflorum mRNA (ADO۱۶۲۴۳.۱, ۸۸%), ۴CL۴ Ocimum tenuiflorum mRNA (ADO۱۶۲۴۲.۱, ۱۰۰%), and CYP۷۶ hydroxylase Withania somnifera mRNA (AGT۰۳۷۷۶.۱, ۱۰۰%) respectively. Bioinformatics information about these enzymes could help to a better understanding of their functions.

Keywords:

Cinnamate ۴-hydroxylase , ۴-coumaroyl CoA-ligase , ۴-Coumarate ۳-hydroxylase , phenylpropanoids