Royal Jelly Decreases MMP-۹ Expression and Induces Apoptosis in Human Bladder Cancer ۵۶۳۷ Cells

Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JCMR-13-1_005

تاریخ نمایه سازی: 22 آذر 1400

Abstract:

Royal jelly (RJ) from queen honeybee larva as a traditional medicine agent has a variety of pharmacological benefits. In the present study, the effect of Royal jelly was investigated on the urinary bladder cancer cell line (HTB-۹ ۵۶۳۷). To determine the cell viability in different concentrations of Royal jelly, MTT assay was performed. An in vitro wound healing assay was applied to investigate the effect of RJ on cell migration. The activity and gene expression level of matrix metalloproteinase ۲ and ۹ was assessed by zymography and Real time PCR respectively. R.J.S at the concentration of ۰.۷ mg/ml had a significant effect on reducing the proliferation rate of ۵۶۳۷ cells after ۷۲h (p < ۰.۰۰۹). R.J.S significantly decreased cell migration and induced a significant decrease in the transcriptional level of MMP۹ after ۷۲h (۰.۵x; P < ۰.۰۴۹). However R.J.S did not impose any effect on the expression level and activity of matrix metalloproteinase ۲. These results indicate the potential of RJ as a promised natural anti-proliferative and anti-metastatic drug in combination with advanced therapy methods for cancer treatment. Royal jelly has the potential to be more focused as an anti-metastatic drug to control tumor growth and can be considered as a more effective alternative to the current chemotherapy drugs.

Authors

Narges Fazili

Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Zahra-Soheila Soheili

Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Saeid Malekzadeh-Shafaroudi

Department of Biotechnology and Plat Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Shahram Samiei

Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

Shamila D.Alipoor

Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Nasrin Moshtaghi

Department of Biotechnology and Plat Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abouzar Bagheri

Department of Clinical Biochemistry and Genetics, Mazandaran University of Medical Science, Iran

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