Sequence analysis reveals asymptomatic infection with Mycoplasma hominis and Ureaplasma urealyticum possibly leads to infertility in females: A cross-sectional study
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJRM-19-11_001
تاریخ نمایه سازی: 24 آذر 1400
Abstract:
Background: Genetic evidence of asymptomatic Mycoplasma hominis and Ureaplasma urealyticum infection associated with infertility among females is lacking because suitable high throughput molecular methods have not been applied.
Objective: This study aimed to explore the occurrence of M. hominis and U. urealyticum in the genital tract of females with asymptomatic infection and infertility as well as determine their genetic relatedness.
Materials and Methods: The study group included ۱۰۰ asymptomatic females and ۳۱ females diagnosed with infertility. Sequencing of the ۱۶S rRNA gene following DNA extraction was performed directly from endo-cervical swabs. Phylogenetic analysis established the genetic linkage between the isolates from both groups.
Results: In asymptomatic females, M. hominis and U. urealyticum were detected with a prevalence of ۸% and ۲% respectively. Among females with infertility, the prevalence was ۶.۴۵% and ۳.۲۳% for M. hominis and U. urealyticum respectively. In both groups, M. hominis occurred significantly more frequently. Phylogenetic analysis revealed three distinct clusters in both groups: two with already characterized M. hominis and Ureaplasma species (۲۸.۶% of the overall Mycoplasma spp.) and one distinct cluster matched with U. urealyticum. Furthermore, all M. hominis from asymptomatic females clustered significantly with infertility contrary to U. urealyticum. The M. hominis cluster was significantly linked to two strains from China.
Conclusion: The sequence analysis of Mycoplasma and Ureaplasma in the genital tract of asymptomatic and infertile females showed significant association; therefore, it is paramount to consider them as possible etiologic agents of infertility and genital infection especially when the etiology of infertility is unknown.
Keywords:
Mycoplasma hominis , Ureaplasma urealyticum , Genetic linkage , Asymptomatic infections , Infertility. , مایکوپلاسما هومینیس , اوره آپلاسما اوره لیتیکوم , پیوند ژنتیکی , عفونتهای بدون علامت , ناباروری.
Authors
Chinyere Charity Ezeanya-Bakpa
Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria. Department of Microbiology and Biotechnology, Caleb University Lagos, Nigeria.
Nneka Regina Agbakoba
Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria.
Charolette Blanche Oguejiofor
Department of Obstetrics and Gynaecology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria.
Ifeoma Bessie Enweani-Nwokelo
Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria.
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