Background and aims: Soluble or circulating form of
Klotho (i.e., anti-aging and longevity protein) has biological effects on various bodycells and tissues. Considering the role of exercise training on protective proteins, the present study aimed to investigate and compare theeffects of short-term high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on plasma levels of Klotho,total antioxidant capacity (TAC), and malondialdehyde (MDA).Methods: In this study, ۲۴ male Wistar rats with a weight range of ۲۵۰-۳۰۰ g and ۸-۱۰ weeks old were randomly divided into control, HIIT,and MICT groups. Training included five consecutive days on the treadmill. HIIT including ۶×۲ minutes high intermittence with ۸۵%-۹۰%Vo۲max and ۵×۲ minutes slow intermittence (active recovery) with ۵۵%-۶۰% Vo۲max. MICT program was performed at ۷۰% Vo۲max thattotal distance running was the same for the HIIT group. Then, the plasma levels of Klotho, TAC, and MDA were measured by ELISA assay.Finally, one-way ANOVA and post-hoc Tukey tests were used for statistical analysis.Results: Based on the results, a significant increase was observed in plasma levels of
Klotho and TAC in both HIIT and MICT groupscompared to the control group (P < ۰.۰۰۱) while these changes were significantly more dominant in the HIIT group compared to MICTgroup (P = ۰.۰۲۴). However, the results demonstrated no significant difference between the groups regarding MDA (P = ۰.۱۰۹). Contrarily,the finding revealed a significant positive correlation between
Klotho and TAC (r=۰.۷۹, ۰.۸۳, ۰.۷۹ in control, HIIT, and MICT groups,respectively, P < ۰.۰۵), whereas a significant negative association between
Klotho with MDA within all the groups (r= -۰.۷۸, - ۰.۸۶, and-۰.۸۱).Conclusion: In general, even short-term aerobic exercise training, especially HIIT increases circulating
Klotho and TAC, therefore, this canconfirm the positive effects of the training-induced exercise.